Flow cytometry is based on the detection of the absorption and diffraction of light at certain angles and the differential evaluation of the signals received. Current instruments operate with laser lights at certain wavelengths, which can guarantee the constancy of the light source. Each stream of cells suspended in solutions in which reagents chosen to identify distinct characteristics are premixed is passed through a thin optical glass cell so that the cells are aligned in a single row during the transit. The beam of light passing through each cell is partly absorbed (photons passing through the cell) and partly deflected (scattering photons impinging at an angle on the cell or being deflected by structures within the cell, such as the nucleus and granules) (Fig. 1). The detection of the magnitude of absorption and the signal from different angular degrees of deflection are useful elements to diversify individual classes of cells by number, size, and peculiar characteristics (Fig. 2). Flow cytometry is widely used in modern hematological instruments in association with electrical impedance so as to guarantee high counting accuracy and allow correct detection of all the cellular elements of the blood, including both immature elements and elements found in hematological disorders (Fig. 3).

Fig1. Light scattering is observed when a beam of light impinges on a cell without being absorbed. Part of the photons are deflected with a low angular degree (forward scattering) in relation to the size of the cell, part is deflected with a high angular degree (side scattering) in relation to the contents inside the cell. Appropriate detectors collect the signal obtained from the cell flow. (Copyright EDISES 2021. Reproduced with permission)

Fig2. Flow cytometer scheme. Each cell passing through the laser light beam generates different signals that are collected by appropriate detectors. The combination of the signals, through a special algorithm, allows each cell to be assigned to its own distinctive class. (Copyright EDISES 2021. Reproduced with permission)

Fig3. Scattering graph. Each cell class is reported in the graph to the area identified by the intersection of signals from FSC-H (forward scattering) and SSC-H (side scattering). The graduation of colors within each area is in relation to the cell density

اخر الاخبار

اخبار العتبة العباسية المقدسة

قسم الشؤون الدينية يُطلق دورة فقهية دينية لملاكات مستشفى الكفيل

شعبة فاطمة بنت أسد للدراسات القرآنية تعلن نتائج مسابقة (ميثاق الرسول)

شعبة الخطابة الحسينية النسوية تنظّم مسابقة فرقية لطالبات برنامج ثمار العطاء

قسم العلاقات ينظم ورشة عن فن الإقناع والتواصل الفعّال للطلبة المنسقين

المزيد

الآخبار الصحية

تغيير بسيط في نمط التفكير قد يحدث فرقا كبيرا لصحة الدماغ ؟

لماذا ينعشنا المشروب الساخن في الطقس الحار؟

علماء يكتشفون فئة دم فريدة من نوعها في العالم !

لماذا ينصح الأطباء بزيت الزيتون يوميا؟

المزيد

الآخبار التكنلوجية

حساس الإطارات.. ما هو وعلامات تلفه وكيفية صيانته؟

ما معنى أودي Quattro؟ سر الأداء والتميز الهندسي ؟!

فصل الشاحن أم الهاتف أولا؟.. عادات بسيطة تطيل عمر البطارية

تعود للقرن الرابع قبل الميلاد.. اكتشاف بقايا "إيمت" المصرية

المزيد

مواضيع ذات صلة

lactic acid (Lactate)

ischemia-modified albumin (IMA)

iron level and total iron-binding capacity (Fe and TIBC, Transferrin saturation, Transferrin)

Platelet Indices and Platelet Count

Red Cell Distribution

intrinsic factor antibody (IF ab)

insulin-like growth factor (IGF-1, Somatomedin C, Insulin-like growth factor binding proteins [IGF BP])

insulin assay

immunoglobulin quantification

Diagnosis of Familial Dyslipidemia

Lipid Profile

5-hydroxyindoleacetic acid (5-HIAA)