Gel-Permeation Chromatography
Gel-permeation chromatography (GPC) is a size-exclusion method that usesor ganic solvents (or bu¤ers) and porous gels for the separation of macromolec ules. The packing gel is characterized by pore size and exclusion range,which must be larger than the analytes of interest. GPC is recommended for
the elimination of lipids, proteins, polymers, copolymers, natural resin s, ce llular components, viruses, steroids, and dispersed high-molecular-weightcom pounds from the sample. This method is appropriate for both polar andnonpolar analytes. Therefore, it is used for extracts containing a broad rangeof analytes. Usually, GPC is most efficient for removing high-boiling materials that condense in the injection port of a GC or the front of the GC column.